Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP

We have developed a rapid and specific method for site-specific 5-hydroxymethylcytosine (5hmC) quantification at single-base resolution. This bisulfite-free method integrates the peroxotungstate oxidization with the mismatched probe-assisted ligation to guarantee the specificity. Moreover, the high-...

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Detalles Bibliográficos
Autores principales: Zhang, Zhenhao, He, Tong, Qi, Yan, Dai, Yuxuan, Lao, Kejing, Gou, Xingchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9261916/
https://www.ncbi.nlm.nih.gov/pubmed/35865199
http://dx.doi.org/10.1039/d2ra03310k
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author Zhang, Zhenhao
He, Tong
Qi, Yan
Dai, Yuxuan
Lao, Kejing
Gou, Xingchun
author_facet Zhang, Zhenhao
He, Tong
Qi, Yan
Dai, Yuxuan
Lao, Kejing
Gou, Xingchun
author_sort Zhang, Zhenhao
collection PubMed
description We have developed a rapid and specific method for site-specific 5-hydroxymethylcytosine (5hmC) quantification at single-base resolution. This bisulfite-free method integrates the peroxotungstate oxidization with the mismatched probe-assisted ligation to guarantee the specificity. Moreover, the high-efficiency LAMP also makes the proposed method suitable for the detection of low-content samples.
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spelling pubmed-92619162022-07-20 Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP Zhang, Zhenhao He, Tong Qi, Yan Dai, Yuxuan Lao, Kejing Gou, Xingchun RSC Adv Chemistry We have developed a rapid and specific method for site-specific 5-hydroxymethylcytosine (5hmC) quantification at single-base resolution. This bisulfite-free method integrates the peroxotungstate oxidization with the mismatched probe-assisted ligation to guarantee the specificity. Moreover, the high-efficiency LAMP also makes the proposed method suitable for the detection of low-content samples. The Royal Society of Chemistry 2022-07-07 /pmc/articles/PMC9261916/ /pubmed/35865199 http://dx.doi.org/10.1039/d2ra03310k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Zhang, Zhenhao
He, Tong
Qi, Yan
Dai, Yuxuan
Lao, Kejing
Gou, Xingchun
Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP
title Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP
title_full Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP
title_fullStr Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP
title_full_unstemmed Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP
title_short Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP
title_sort rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based lamp
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9261916/
https://www.ncbi.nlm.nih.gov/pubmed/35865199
http://dx.doi.org/10.1039/d2ra03310k
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