Direct tracking of reverse-transcriptase speed and template sensitivity: implications for sequencing and analysis of long RNA molecules

Although reverse-transcriptase (RT) enzymes are critical reagents for research and biotechnology, their mechanical properties are not well understood. In particular, we know little about their relative speed and response to structural obstacles in the template. Commercial retroviral RTs stop at many...

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Detalles Bibliográficos
Autores principales: Guo, Li-Tao, Olson, Sara, Patel, Shivali, Graveley, Brenton R, Pyle, Anna Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262592/
https://www.ncbi.nlm.nih.gov/pubmed/35713547
http://dx.doi.org/10.1093/nar/gkac518

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262592/
https://www.ncbi.nlm.nih.gov/pubmed/35713547
http://dx.doi.org/10.1093/nar/gkac518

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