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Direct tracking of reverse-transcriptase speed and template sensitivity: implications for sequencing and analysis of long RNA molecules
Although reverse-transcriptase (RT) enzymes are critical reagents for research and biotechnology, their mechanical properties are not well understood. In particular, we know little about their relative speed and response to structural obstacles in the template. Commercial retroviral RTs stop at many...
Autores principales: | Guo, Li-Tao, Olson, Sara, Patel, Shivali, Graveley, Brenton R, Pyle, Anna Marie |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262592/ https://www.ncbi.nlm.nih.gov/pubmed/35713547 http://dx.doi.org/10.1093/nar/gkac518 |
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