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Oxford Nanopore R10.4 long-read sequencing enables the generation of near-finished bacterial genomes from pure cultures and metagenomes without short-read or reference polishing

Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include short-read polishing to correct insertions and del...

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Detalles Bibliográficos
Autores principales: Sereika, Mantas, Kirkegaard, Rasmus Hansen, Karst, Søren Michael, Michaelsen, Thomas Yssing, Sørensen, Emil Aarre, Wollenberg, Rasmus Dam, Albertsen, Mads
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262707/
https://www.ncbi.nlm.nih.gov/pubmed/35789207
http://dx.doi.org/10.1038/s41592-022-01539-7