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In vitro fermentation profiles of different soybean oligosaccharides and their effects on skatole production and cecal microbiota of broilers

OBJECTIVE: The objective of this study was to investigate the in vitro fermentation profiles of different soybean oligosaccharides (SBOs) and their effects on skatole production and cecal microbiota of broilers. METHODS: Five SBOs with varying main component contents were fermented using an in vitro...

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Autores principales: Zhu, Xin, Xu, Miao, Liu, Haiying, Yang, Guiqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Animal Bioscience 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262728/
https://www.ncbi.nlm.nih.gov/pubmed/34991192
http://dx.doi.org/10.5713/ab.21.0424
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author Zhu, Xin
Xu, Miao
Liu, Haiying
Yang, Guiqin
author_facet Zhu, Xin
Xu, Miao
Liu, Haiying
Yang, Guiqin
author_sort Zhu, Xin
collection PubMed
description OBJECTIVE: The objective of this study was to investigate the in vitro fermentation profiles of different soybean oligosaccharides (SBOs) and their effects on skatole production and cecal microbiota of broilers. METHODS: Five SBOs with varying main component contents were fermented using an in vitro batch incubation inoculated with broiler cecal microbiota. Gas production was recorded automatically, skatole, indole and short-chain fatty acids (SCFAs) were determined using high-performance liquid chromatography, and microbial changes were analyzed using 16S DNA gene sequencing. RESULTS: The addition of SBOs increased (p<0.05) gas production, suggesting bacterial growth-stimulating activities. In addition, the concentrations of indole were significantly (p<0.05) decreased after SBO supplementation, and SBO III, with higher sucrose and stachyose contents, decreased (p<0.05) the skatole level. Our results also revealed that the fermentation of SBOs by cecal microbiota produced (p<0.05) SCFAs, which were dominated by propionic acid, butyrate acid and lactic acid compared to the control. In addition, SBO III increased (p<0.05) the abundance of Firmicutes and Subdoligranulum and decreased that of Bacteroides. CONCLUSION: These results suggest that SBOs with higher sucrose and stachyose contents are promising prebiotics in modulating gut microbiota and reducing odor emission in broilers.
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spelling pubmed-92627282022-08-01 In vitro fermentation profiles of different soybean oligosaccharides and their effects on skatole production and cecal microbiota of broilers Zhu, Xin Xu, Miao Liu, Haiying Yang, Guiqin Anim Biosci Article OBJECTIVE: The objective of this study was to investigate the in vitro fermentation profiles of different soybean oligosaccharides (SBOs) and their effects on skatole production and cecal microbiota of broilers. METHODS: Five SBOs with varying main component contents were fermented using an in vitro batch incubation inoculated with broiler cecal microbiota. Gas production was recorded automatically, skatole, indole and short-chain fatty acids (SCFAs) were determined using high-performance liquid chromatography, and microbial changes were analyzed using 16S DNA gene sequencing. RESULTS: The addition of SBOs increased (p<0.05) gas production, suggesting bacterial growth-stimulating activities. In addition, the concentrations of indole were significantly (p<0.05) decreased after SBO supplementation, and SBO III, with higher sucrose and stachyose contents, decreased (p<0.05) the skatole level. Our results also revealed that the fermentation of SBOs by cecal microbiota produced (p<0.05) SCFAs, which were dominated by propionic acid, butyrate acid and lactic acid compared to the control. In addition, SBO III increased (p<0.05) the abundance of Firmicutes and Subdoligranulum and decreased that of Bacteroides. CONCLUSION: These results suggest that SBOs with higher sucrose and stachyose contents are promising prebiotics in modulating gut microbiota and reducing odor emission in broilers. Animal Bioscience 2022-08 2022-01-05 /pmc/articles/PMC9262728/ /pubmed/34991192 http://dx.doi.org/10.5713/ab.21.0424 Text en Copyright © 2022 by Animal Bioscience https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Zhu, Xin
Xu, Miao
Liu, Haiying
Yang, Guiqin
In vitro fermentation profiles of different soybean oligosaccharides and their effects on skatole production and cecal microbiota of broilers
title In vitro fermentation profiles of different soybean oligosaccharides and their effects on skatole production and cecal microbiota of broilers
title_full In vitro fermentation profiles of different soybean oligosaccharides and their effects on skatole production and cecal microbiota of broilers
title_fullStr In vitro fermentation profiles of different soybean oligosaccharides and their effects on skatole production and cecal microbiota of broilers
title_full_unstemmed In vitro fermentation profiles of different soybean oligosaccharides and their effects on skatole production and cecal microbiota of broilers
title_short In vitro fermentation profiles of different soybean oligosaccharides and their effects on skatole production and cecal microbiota of broilers
title_sort in vitro fermentation profiles of different soybean oligosaccharides and their effects on skatole production and cecal microbiota of broilers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262728/
https://www.ncbi.nlm.nih.gov/pubmed/34991192
http://dx.doi.org/10.5713/ab.21.0424
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