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Counting fluorescently labeled proteins in tissues in the spinning–disk microscope using single–molecule calibrations
Quantification of molecular numbers and concentrations in living cells is critical for testing models of complex biological phenomena. Counting molecules in cells requires estimation of the fluorescence intensity of single molecules, which is generally limited to imaging near cell surfaces, in isola...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The American Society for Cell Biology
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265152/ https://www.ncbi.nlm.nih.gov/pubmed/35323029 http://dx.doi.org/10.1091/mbc.E21-12-0618 |