Cargando…
Standardization of Cell Culture Conditions and Routine Genomic Screening under a Quality Management System Leads to Reduced Genomic Instability in hPSCs
Human pluripotent stem cells (hPSCs) have generated unprecedented interest in the scientific community, given their potential applications in regenerative medicine, disease modeling, toxicology and drug screening. However, hPSCs are prone to acquire genomic alterations in vitro, mainly due to subopt...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265327/ https://www.ncbi.nlm.nih.gov/pubmed/35805069 http://dx.doi.org/10.3390/cells11131984 |
_version_ | 1784743186115067904 |
---|---|
author | Molina-Ruiz, Francisco J. Introna, Clelia Bombau, Georgina Galofre, Mireia Canals, Josep M. |
author_facet | Molina-Ruiz, Francisco J. Introna, Clelia Bombau, Georgina Galofre, Mireia Canals, Josep M. |
author_sort | Molina-Ruiz, Francisco J. |
collection | PubMed |
description | Human pluripotent stem cells (hPSCs) have generated unprecedented interest in the scientific community, given their potential applications in regenerative medicine, disease modeling, toxicology and drug screening. However, hPSCs are prone to acquire genomic alterations in vitro, mainly due to suboptimal culture conditions and inappropriate routines to monitor genome integrity. This poses a challenge to both the safety of clinical applications and the reliability of basic and translational hPSC research. In this study, we aim to investigate if the implementation of a Quality Management System (QMS) such as ISO9001:2015 to ensure reproducible and standardized cell culture conditions and genomic screening strategies can decrease the prevalence of genomic alterations affecting hPSCs used for research applications. To this aim, we performed a retrospective analysis of G-banding karyotype and Comparative Genomic Hybridization array (aCGH) data generated by our group over a 5-year span of different hESC and hiPSC cultures. This work demonstrates that application of a QMS to standardize cell culture conditions and genomic monitoring routines leads to a striking improvement of genomic stability in hPSCs cultured in vitro, as evidenced by a reduced probability of potentially pathogenic chromosomal aberrations and subchromosomal genomic alterations. These results support the need to implement QMS in academic laboratories performing hPSC research. |
format | Online Article Text |
id | pubmed-9265327 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92653272022-07-09 Standardization of Cell Culture Conditions and Routine Genomic Screening under a Quality Management System Leads to Reduced Genomic Instability in hPSCs Molina-Ruiz, Francisco J. Introna, Clelia Bombau, Georgina Galofre, Mireia Canals, Josep M. Cells Article Human pluripotent stem cells (hPSCs) have generated unprecedented interest in the scientific community, given their potential applications in regenerative medicine, disease modeling, toxicology and drug screening. However, hPSCs are prone to acquire genomic alterations in vitro, mainly due to suboptimal culture conditions and inappropriate routines to monitor genome integrity. This poses a challenge to both the safety of clinical applications and the reliability of basic and translational hPSC research. In this study, we aim to investigate if the implementation of a Quality Management System (QMS) such as ISO9001:2015 to ensure reproducible and standardized cell culture conditions and genomic screening strategies can decrease the prevalence of genomic alterations affecting hPSCs used for research applications. To this aim, we performed a retrospective analysis of G-banding karyotype and Comparative Genomic Hybridization array (aCGH) data generated by our group over a 5-year span of different hESC and hiPSC cultures. This work demonstrates that application of a QMS to standardize cell culture conditions and genomic monitoring routines leads to a striking improvement of genomic stability in hPSCs cultured in vitro, as evidenced by a reduced probability of potentially pathogenic chromosomal aberrations and subchromosomal genomic alterations. These results support the need to implement QMS in academic laboratories performing hPSC research. MDPI 2022-06-21 /pmc/articles/PMC9265327/ /pubmed/35805069 http://dx.doi.org/10.3390/cells11131984 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Molina-Ruiz, Francisco J. Introna, Clelia Bombau, Georgina Galofre, Mireia Canals, Josep M. Standardization of Cell Culture Conditions and Routine Genomic Screening under a Quality Management System Leads to Reduced Genomic Instability in hPSCs |
title | Standardization of Cell Culture Conditions and Routine Genomic Screening under a Quality Management System Leads to Reduced Genomic Instability in hPSCs |
title_full | Standardization of Cell Culture Conditions and Routine Genomic Screening under a Quality Management System Leads to Reduced Genomic Instability in hPSCs |
title_fullStr | Standardization of Cell Culture Conditions and Routine Genomic Screening under a Quality Management System Leads to Reduced Genomic Instability in hPSCs |
title_full_unstemmed | Standardization of Cell Culture Conditions and Routine Genomic Screening under a Quality Management System Leads to Reduced Genomic Instability in hPSCs |
title_short | Standardization of Cell Culture Conditions and Routine Genomic Screening under a Quality Management System Leads to Reduced Genomic Instability in hPSCs |
title_sort | standardization of cell culture conditions and routine genomic screening under a quality management system leads to reduced genomic instability in hpscs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265327/ https://www.ncbi.nlm.nih.gov/pubmed/35805069 http://dx.doi.org/10.3390/cells11131984 |
work_keys_str_mv | AT molinaruizfranciscoj standardizationofcellcultureconditionsandroutinegenomicscreeningunderaqualitymanagementsystemleadstoreducedgenomicinstabilityinhpscs AT intronaclelia standardizationofcellcultureconditionsandroutinegenomicscreeningunderaqualitymanagementsystemleadstoreducedgenomicinstabilityinhpscs AT bombaugeorgina standardizationofcellcultureconditionsandroutinegenomicscreeningunderaqualitymanagementsystemleadstoreducedgenomicinstabilityinhpscs AT galofremireia standardizationofcellcultureconditionsandroutinegenomicscreeningunderaqualitymanagementsystemleadstoreducedgenomicinstabilityinhpscs AT canalsjosepm standardizationofcellcultureconditionsandroutinegenomicscreeningunderaqualitymanagementsystemleadstoreducedgenomicinstabilityinhpscs |