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Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice

This study aims to determine the immunomodulatory effects of a polysaccharide fraction from fermented M. citrifolia L. (FMP) in RAW 264.7 macrophages and Balb/c mice. M. citrifolia was fermented for 72 h using Lactobacillus brevis; polysaccharides were extracted using ethanol precipitation. The RAW...

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Autores principales: Choi, Sun-Il, La, Im-Joung, Han, Xionggao, Men, Xiao, Lee, Se-Jeong, Oh, Geon, Kwon, Hee-Yeon, Kim, Yong-Deok, Seong, Geum-Su, Kim, Seung-Hyung, Lee, Ok-Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266266/
https://www.ncbi.nlm.nih.gov/pubmed/35804740
http://dx.doi.org/10.3390/foods11131925
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author Choi, Sun-Il
La, Im-Joung
Han, Xionggao
Men, Xiao
Lee, Se-Jeong
Oh, Geon
Kwon, Hee-Yeon
Kim, Yong-Deok
Seong, Geum-Su
Kim, Seung-Hyung
Lee, Ok-Hwan
author_facet Choi, Sun-Il
La, Im-Joung
Han, Xionggao
Men, Xiao
Lee, Se-Jeong
Oh, Geon
Kwon, Hee-Yeon
Kim, Yong-Deok
Seong, Geum-Su
Kim, Seung-Hyung
Lee, Ok-Hwan
author_sort Choi, Sun-Il
collection PubMed
description This study aims to determine the immunomodulatory effects of a polysaccharide fraction from fermented M. citrifolia L. (FMP) in RAW 264.7 macrophages and Balb/c mice. M. citrifolia was fermented for 72 h using Lactobacillus brevis; polysaccharides were extracted using ethanol precipitation. The RAW 264.7 cells exposed to FMP (50, 100, and 200 μg/mL) for 24 h showed increased NO production, proinflammatory cytokine (IL-1β, IL-6, and TNF-α) release, and COX-2 and iNOS protein expression. FMP (100, 200 mg/kg) and deacetylasperulosidic acid (DAA) (20 mg/kg) administered orally to Balb/c mice for 14 days upregulated NO production and NK cytotoxicity in abdominal cavity and spleen, respectively. Th1 and Th2 cytokines production and immune cell numbers increased in spleen, mesenteric lymph nodes (MLN), peritoneal exudate cells (PEC), Peyer’s patches (PP), and peripheral blood mononuclear cells (PBMC). Therefore, FMP containing DAA can be used as materials for health functional foods to enhance immune responses.
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spelling pubmed-92662662022-07-09 Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice Choi, Sun-Il La, Im-Joung Han, Xionggao Men, Xiao Lee, Se-Jeong Oh, Geon Kwon, Hee-Yeon Kim, Yong-Deok Seong, Geum-Su Kim, Seung-Hyung Lee, Ok-Hwan Foods Article This study aims to determine the immunomodulatory effects of a polysaccharide fraction from fermented M. citrifolia L. (FMP) in RAW 264.7 macrophages and Balb/c mice. M. citrifolia was fermented for 72 h using Lactobacillus brevis; polysaccharides were extracted using ethanol precipitation. The RAW 264.7 cells exposed to FMP (50, 100, and 200 μg/mL) for 24 h showed increased NO production, proinflammatory cytokine (IL-1β, IL-6, and TNF-α) release, and COX-2 and iNOS protein expression. FMP (100, 200 mg/kg) and deacetylasperulosidic acid (DAA) (20 mg/kg) administered orally to Balb/c mice for 14 days upregulated NO production and NK cytotoxicity in abdominal cavity and spleen, respectively. Th1 and Th2 cytokines production and immune cell numbers increased in spleen, mesenteric lymph nodes (MLN), peritoneal exudate cells (PEC), Peyer’s patches (PP), and peripheral blood mononuclear cells (PBMC). Therefore, FMP containing DAA can be used as materials for health functional foods to enhance immune responses. MDPI 2022-06-28 /pmc/articles/PMC9266266/ /pubmed/35804740 http://dx.doi.org/10.3390/foods11131925 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Choi, Sun-Il
La, Im-Joung
Han, Xionggao
Men, Xiao
Lee, Se-Jeong
Oh, Geon
Kwon, Hee-Yeon
Kim, Yong-Deok
Seong, Geum-Su
Kim, Seung-Hyung
Lee, Ok-Hwan
Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice
title Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice
title_full Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice
title_fullStr Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice
title_full_unstemmed Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice
title_short Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice
title_sort immunomodulatory effect of polysaccharide from fermented morinda citrifolia l. (noni) on raw 264.7 macrophage and balb/c mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266266/
https://www.ncbi.nlm.nih.gov/pubmed/35804740
http://dx.doi.org/10.3390/foods11131925
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