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Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice
This study aims to determine the immunomodulatory effects of a polysaccharide fraction from fermented M. citrifolia L. (FMP) in RAW 264.7 macrophages and Balb/c mice. M. citrifolia was fermented for 72 h using Lactobacillus brevis; polysaccharides were extracted using ethanol precipitation. The RAW...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266266/ https://www.ncbi.nlm.nih.gov/pubmed/35804740 http://dx.doi.org/10.3390/foods11131925 |
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author | Choi, Sun-Il La, Im-Joung Han, Xionggao Men, Xiao Lee, Se-Jeong Oh, Geon Kwon, Hee-Yeon Kim, Yong-Deok Seong, Geum-Su Kim, Seung-Hyung Lee, Ok-Hwan |
author_facet | Choi, Sun-Il La, Im-Joung Han, Xionggao Men, Xiao Lee, Se-Jeong Oh, Geon Kwon, Hee-Yeon Kim, Yong-Deok Seong, Geum-Su Kim, Seung-Hyung Lee, Ok-Hwan |
author_sort | Choi, Sun-Il |
collection | PubMed |
description | This study aims to determine the immunomodulatory effects of a polysaccharide fraction from fermented M. citrifolia L. (FMP) in RAW 264.7 macrophages and Balb/c mice. M. citrifolia was fermented for 72 h using Lactobacillus brevis; polysaccharides were extracted using ethanol precipitation. The RAW 264.7 cells exposed to FMP (50, 100, and 200 μg/mL) for 24 h showed increased NO production, proinflammatory cytokine (IL-1β, IL-6, and TNF-α) release, and COX-2 and iNOS protein expression. FMP (100, 200 mg/kg) and deacetylasperulosidic acid (DAA) (20 mg/kg) administered orally to Balb/c mice for 14 days upregulated NO production and NK cytotoxicity in abdominal cavity and spleen, respectively. Th1 and Th2 cytokines production and immune cell numbers increased in spleen, mesenteric lymph nodes (MLN), peritoneal exudate cells (PEC), Peyer’s patches (PP), and peripheral blood mononuclear cells (PBMC). Therefore, FMP containing DAA can be used as materials for health functional foods to enhance immune responses. |
format | Online Article Text |
id | pubmed-9266266 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92662662022-07-09 Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice Choi, Sun-Il La, Im-Joung Han, Xionggao Men, Xiao Lee, Se-Jeong Oh, Geon Kwon, Hee-Yeon Kim, Yong-Deok Seong, Geum-Su Kim, Seung-Hyung Lee, Ok-Hwan Foods Article This study aims to determine the immunomodulatory effects of a polysaccharide fraction from fermented M. citrifolia L. (FMP) in RAW 264.7 macrophages and Balb/c mice. M. citrifolia was fermented for 72 h using Lactobacillus brevis; polysaccharides were extracted using ethanol precipitation. The RAW 264.7 cells exposed to FMP (50, 100, and 200 μg/mL) for 24 h showed increased NO production, proinflammatory cytokine (IL-1β, IL-6, and TNF-α) release, and COX-2 and iNOS protein expression. FMP (100, 200 mg/kg) and deacetylasperulosidic acid (DAA) (20 mg/kg) administered orally to Balb/c mice for 14 days upregulated NO production and NK cytotoxicity in abdominal cavity and spleen, respectively. Th1 and Th2 cytokines production and immune cell numbers increased in spleen, mesenteric lymph nodes (MLN), peritoneal exudate cells (PEC), Peyer’s patches (PP), and peripheral blood mononuclear cells (PBMC). Therefore, FMP containing DAA can be used as materials for health functional foods to enhance immune responses. MDPI 2022-06-28 /pmc/articles/PMC9266266/ /pubmed/35804740 http://dx.doi.org/10.3390/foods11131925 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Choi, Sun-Il La, Im-Joung Han, Xionggao Men, Xiao Lee, Se-Jeong Oh, Geon Kwon, Hee-Yeon Kim, Yong-Deok Seong, Geum-Su Kim, Seung-Hyung Lee, Ok-Hwan Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice |
title | Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice |
title_full | Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice |
title_fullStr | Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice |
title_full_unstemmed | Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice |
title_short | Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice |
title_sort | immunomodulatory effect of polysaccharide from fermented morinda citrifolia l. (noni) on raw 264.7 macrophage and balb/c mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266266/ https://www.ncbi.nlm.nih.gov/pubmed/35804740 http://dx.doi.org/10.3390/foods11131925 |
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