Cargando…

Thermal Shift Assay for Small GTPase Stability Screening: Evaluation and Suitability

Thermal unfolding methods are commonly used as a predictive technique by tracking the protein’s physical properties. Inherent protein thermal stability and unfolding profiles of biotherapeutics can help to screen or study potential drugs and to find stabilizing or destabilizing conditions. Different...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kopra, Kari, Valtonen, Salla, Mahran, Randa, Kapp, Jonas N., Hassan, Nazia, Gillette, William, Dennis, Bryce, Li, Lianbo, Westover, Kenneth D., Plückthun, Andreas, Härmä, Harri
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266822/ https://www.ncbi.nlm.nih.gov/pubmed/35806100 http://dx.doi.org/10.3390/ijms23137095

Ejemplares similares

Protease Substrate-Independent Universal Assay for Monitoring Digestion of Native Unmodified Proteins
por: Vuorinen, Emmiliisa, et al.
Publicado: (2021)

Thermal Dissociation Assay for Time-Resolved Fluorescence Detection of Protein Post-Translational Modifications
por: Eskonen, Ville, et al.
Publicado: (2019)

Sensitive, homogeneous, and label-free protein-probe assay for antibody aggregation and thermal stability studies
por: Valtonen, Salla, et al.
Publicado: (2021)

Sensitive Label-Free Thermal Stability Assay for Protein Denaturation and Protein–Ligand Interaction Studies
por: Vuorinen, Emmiliisa, et al.
Publicado: (2020)

Isothermal chemical denaturation assay for monitoring protein stability and inhibitor interactions
por: Mahran, Randa, et al.
Publicado: (2023)

Rapid high-throughput compatible label-free virus particle quantification method based on time-resolved luminescence
por: Kopra, Kari, et al.
Publicado: (2022)

Nanomolar Protein–Protein Interaction Monitoring with a Label-Free Protein-Probe Technique
por: Valtonen, Salla, et al.
Publicado: (2020)

Homogeneous luminescent quantitation of cellular guanosine and adenosine triphosphates (GTP and ATP) using QT-Luc(GTP&ATP) assay
por: Kopra, Kari, et al.
Publicado: (2023)

Homogeneous Dual-Parametric-Coupled Assay for Simultaneous Nucleotide Exchange and KRAS/RAF-RBD Interaction Monitoring
por: Kopra, Kari, et al.
Publicado: (2020)

Homogeneous single-label cGMP detection platform for the functional study of nitric oxide-sensitive (soluble) guanylyl cyclases and cGMP-specific phosphodiesterases
por: Kopra, Kari, et al.
Publicado: (2020)

Label-Free Time-Gated Luminescent Detection Method for the Nucleotides with Varying Phosphate Content
por: Kopra, Kari, et al.
Publicado: (2018)

Single-Peptide TR-FRET Detection Platform for Cysteine-Specific Post-Translational Modifications
por: Eskonen, Ville, et al.
Publicado: (2020)

Dynamics of Cellular Automata on Beta-Shifts and Direct Topological Factorizations
por: Kopra, Johan
Publicado: (2020)

Rapid assessment of DCLK1 inhibitors using a peptide substrate mobility shift assay
por: Liu, Yan, et al.
Publicado: (2021)

Specific cancer-associated mutations in the switch III region of Ras increase tumorigenicity by nanocluster augmentation
por: Šolman, Maja, et al.
Publicado: (2015)

Ovarian Tumor Microenvironment Signaling: Convergence on the Rac1 GTPase
por: Hudson, Laurie G., et al.
Publicado: (2018)

Rab GTPases and the Autophagy Pathway: Bacterial Targets for a Suitable Biogenesis and Trafficking of Their Own Vacuoles
por: López de Armentia, María Milagros, et al.
Publicado: (2016)

Looking for protein stabilizing drugs with thermal shift assay
por: Andreotti, Giuseppina, et al.
Publicado: (2015)

Are phase shift methods suitable for image matching in photogrammetry?
por: Stokes, J
Publicado: (1985)

Cognitive behavioural therapy interventions for insomnia among shift workers: RCT in an occupational health setting
por: Järnefelt, Heli, et al.
Publicado: (2019)

Optimization of protein samples for NMR using thermal shift assays
por: Kozak, Sandra, et al.
Publicado: (2016)

Homogeneous Assay for Target Engagement Utilizing Bioluminescent Thermal Shift
por: Dart, Melanie L., et al.
Publicado: (2018)

A superior loading control for the cellular thermal shift assay
por: Delport, Alexandré, et al.
Publicado: (2022)

The crossover conformational shift of the GTPase atlastin provides the energy driving ER fusion
por: Winsor, James, et al.
Publicado: (2017)

Shifting suitability for malaria vectors across Africa with warming climates
por: Peterson, A Townsend
Publicado: (2009)

Correction to: Cognitive behavioural therapy interventions for insomnia among shift workers: RCT in an occupational health setting
por: Järnefelt, Heli, et al.
Publicado: (2020)

An automated iterative approach for protein structure refinement using pseudocontact shifts
por: Cucuzza, Stefano, et al.
Publicado: (2021)

Shift work with and without night shifts and breast cancer risk in a cohort study from Finland
por: Härmä, Mikko, et al.
Publicado: (2023)

POT1 stability and binding measured by fluorescence thermal shift assays
por: DeLeeuw, Lynn W., et al.
Publicado: (2021)

Real-Time Cellular Thermal Shift Assay to Monitor Target Engagement
por: Sanchez, Tino W., et al.
Publicado: (2022)

A pathway linking oxidative stress and the Ran GTPase system in progeria
por: Datta, Sutirtha, et al.
Publicado: (2014)

Trajectories of part-time work and sickness absence among shift working healthcare employees
por: Ropponen, A, et al.
Publicado: (2022)

EDTA aggregates induce SYPRO orange-based fluorescence in thermal shift assay
por: Kroeger, Tobias, et al.
Publicado: (2017)

Thermal and Electrical Characterization of Polyester Resins Suitable for Electric Motor Insulation
por: Calabrese, Elisa, et al.
Publicado: (2023)

Detection of Chemical Engagement of Solute Carrier Proteins by a Cellular Thermal Shift Assay
por: Hashimoto, Mari, et al.
Publicado: (2018)

Development of NMR and thermal shift assays for the evaluation of Mycobacterium tuberculosis isocitrate lyase inhibitors
por: Bhusal, Ram Prasad, et al.
Publicado: (2017)

Upward Altitudinal Shifts in Habitat Suitability of Mountain Vipers since the Last Glacial Maximum
por: Yousefi, Masoud, et al.
Publicado: (2015)

Geographic shifts in the bioclimatic suitability for Aedes aegypti under climate change scenarios in Colombia
por: Portilla Cabrera, Cristiam Victoriano, et al.
Publicado: (2019)

Empirical estimation of habitat suitability for rare plant restoration in an era of ongoing climatic shifts
por: Krushelnycky, Paul D., et al.
Publicado: (2023)

Suitability of Different Diagnostic Platforms for Virological Testing of Blood Samples from Cornea Donors
por: Kohmer, Niko, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_5omtaqvs3kdivg045a35ln6ct2