Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons

Auxin-inducible degrons are a chemical genetic tool for targeted protein degradation and are widely used to study protein function in cultured mammalian cells. Here, we develop CRISPR-engineered mouse lines that enable rapid and highly specific degradation of tagged endogenous proteins in vivo. Most...

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Detalles Bibliográficos
Autores principales: Macdonald, Lewis, Taylor, Gillian C, Brisbane, Jennifer Margaret, Christodoulou, Ersi, Scott, Lucy, von Kriegsheim, Alex, Rossant, Janet, Gu, Bin, Wood, Andrew J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Chromosomes and Gene Expression
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273210/
https://www.ncbi.nlm.nih.gov/pubmed/35736539
http://dx.doi.org/10.7554/eLife.77987

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273210/
https://www.ncbi.nlm.nih.gov/pubmed/35736539
http://dx.doi.org/10.7554/eLife.77987

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