CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing

Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~ 2 months....

Descripción completa

Detalles Bibliográficos
Autores principales: Feuer, Kyra L., Wahbeh, Marah H., Yovo, Christian, Rabie, Eman, Lam, Anh-Thu N., Abdollahi, Sara, Young, Lindsay J., Rike, Bailey, Umamageswaran, Akul, Avramopoulos, Dimitrios
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279498/
https://www.ncbi.nlm.nih.gov/pubmed/35831384
http://dx.doi.org/10.1038/s41598-022-16004-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279498/
https://www.ncbi.nlm.nih.gov/pubmed/35831384
http://dx.doi.org/10.1038/s41598-022-16004-w

Ejemplares similares