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Naïve Primary Mouse CD8(+) T Cells Retain In Vivo Immune Responsiveness After Electroporation-Based CRISPR/Cas9 Genetic Engineering
CRISPR/Cas9 technology has revolutionized genetic engineering of primary cells. Although its use is gaining momentum in studies on CD8(+) T cell biology, it remains elusive to what extent CRISPR/Cas9 affects in vivo function of CD8(+) T cells. Here, we optimized nucleofection-based CRISPR/Cas9 genet...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280190/ https://www.ncbi.nlm.nih.gov/pubmed/35844563 http://dx.doi.org/10.3389/fimmu.2022.777113 |