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Oleic acid based experimental evolution of Bacillus megaterium yielding an enhanced P450 BM3 variant

BACKGROUND: Unlike most other P450 cytochrome monooxygenases, CYP102A1 from Bacillus megaterium (BM3) is both soluble and fused to its redox partner forming a single polypeptide chain. Like other monooxygenases, it can catalyze the insertion of oxygen unto the carbon-hydrogen bond which can result i...

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Detalles Bibliográficos
Autores principales: Vincent, Thierry, Gaillet, Bruno, Garnier, Alain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281120/
https://www.ncbi.nlm.nih.gov/pubmed/35831844
http://dx.doi.org/10.1186/s12896-022-00750-w