Transient transfection and purification of SARS-CoV-2 spike protein from mammalian cells

SARS-CoV-2 spike (S) protein ectodomain purification can be challenging, with engineered and natural variations often resulting in lower yields. Here, we present a detailed transfection and purification protocol for the SARS-CoV-2 S ectodomain. We describe how to trace protein yields during purifica...

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Detalles Bibliográficos
Autores principales: Stalls, Victoria, Janowska, Katarzyna, Acharya, Priyamvada
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283606/
https://www.ncbi.nlm.nih.gov/pubmed/35983170
http://dx.doi.org/10.1016/j.xpro.2022.101603

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283606/
https://www.ncbi.nlm.nih.gov/pubmed/35983170
http://dx.doi.org/10.1016/j.xpro.2022.101603

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