A joint PCR-based gene-targeting method using electroporation in the pathogenic fungus Trichosporon asahii

Trichosporon asahii is a pathogenic fungus that causes deep-seated fungal infections in immunocompromised patients. Established methods for generating gene-deficient T. asahii mutants exist, but the frequency of obtaining transformants by electroporation remains low. In the present study, we optimiz...

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Detalles Bibliográficos
Autores principales: Matsumoto, Yasuhiko, Nagamachi, Tae, Yoshikawa, Asami, Yamada, Tsuyoshi, Sugita, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283638/
https://www.ncbi.nlm.nih.gov/pubmed/35834071
http://dx.doi.org/10.1186/s13568-022-01431-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283638/
https://www.ncbi.nlm.nih.gov/pubmed/35834071
http://dx.doi.org/10.1186/s13568-022-01431-9

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