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A joint PCR-based gene-targeting method using electroporation in the pathogenic fungus Trichosporon asahii
Trichosporon asahii is a pathogenic fungus that causes deep-seated fungal infections in immunocompromised patients. Established methods for generating gene-deficient T. asahii mutants exist, but the frequency of obtaining transformants by electroporation remains low. In the present study, we optimiz...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Berlin Heidelberg
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283638/ https://www.ncbi.nlm.nih.gov/pubmed/35834071 http://dx.doi.org/10.1186/s13568-022-01431-9 |