Protocol for qPCR analysis that corrects for cDNA amplification efficiency

This protocol presents a variation on the 2(-ΔΔCt) technique for qPCR analysis. Our approach requires the inclusion of a standard curve on each qPCR plate, and like the 2(-ΔΔCt) technique, is dependent on the stability of housekeeping gene expression. However, unlike the 2(-ΔΔCt) technique, our appr...

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Detalles Bibliográficos
Autores principales: Damgaard, Mads V., Treebak, Jonas T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283931/
https://www.ncbi.nlm.nih.gov/pubmed/35819886
http://dx.doi.org/10.1016/j.xpro.2022.101515

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283931/
https://www.ncbi.nlm.nih.gov/pubmed/35819886
http://dx.doi.org/10.1016/j.xpro.2022.101515

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