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Protocol for qPCR analysis that corrects for cDNA amplification efficiency
This protocol presents a variation on the 2(-ΔΔCt) technique for qPCR analysis. Our approach requires the inclusion of a standard curve on each qPCR plate, and like the 2(-ΔΔCt) technique, is dependent on the stability of housekeeping gene expression. However, unlike the 2(-ΔΔCt) technique, our appr...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283931/ https://www.ncbi.nlm.nih.gov/pubmed/35819886 http://dx.doi.org/10.1016/j.xpro.2022.101515 |