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Measurement of autophagy via LC3 western blotting following DNA-damage-induced senescence

Senescent cells accumulation is associated with aging and age-related diseases, and recent findings suggest that autophagy, the activity of the intracellular degradation system, decreases during senescence. In this protocol, we detail steps to induce cellular senescence in response to DNA damage, ev...

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Detalles Bibliográficos
Autores principales: Yamamoto-Imoto, Hitomi, Hara, Eiji, Nakamura, Shuhei, Yoshimori, Tamotsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283947/
https://www.ncbi.nlm.nih.gov/pubmed/35819884
http://dx.doi.org/10.1016/j.xpro.2022.101539