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Insertion Pool Sequencing for Insertional Mutant Analysis in Complex Host‐Microbe Interactions
Insertional mutant libraries of microorganisms can be applied in negative depletion screens to decipher gene functions. Because of underrepresentation in colonized tissue, one major bottleneck is analysis of species that colonize hosts. To overcome this, we developed insertion pool sequencing (iPool...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9285383/ https://www.ncbi.nlm.nih.gov/pubmed/31479207 http://dx.doi.org/10.1002/cppb.20097 |