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A mass spectrometry‐based non‐radioactive differential radial capillary action of ligand assay (DRaCALA) to assess ligand binding to proteins

Binding of ligands to macromolecules changes their physicochemical and enzymatic characteristics. Cyclic di‐GMP is a second messenger involved in motility/sessility and acute/chronic infection life style transition. Although the GGDEF domain, predominantly a diguanylate cyclase, represents one of th...

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Detalles Bibliográficos
Autores principales: Cimdins‐Ahne, Annika, Chernobrovkin, Alexey, Kim, Soo‐Kyoung, Lee, Vincent T., Zubarev, Roman A., Römling, Ute
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9285882/
https://www.ncbi.nlm.nih.gov/pubmed/35362254
http://dx.doi.org/10.1002/jms.4822