Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue

Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform RNA sequencing (SnISOr-Seq). Using microfluidics,...

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Detalles Bibliográficos
Autores principales: Hardwick, Simon A., Hu, Wen, Joglekar, Anoushka, Fan, Li, Collier, Paul G., Foord, Careen, Balacco, Jennifer, Lanjewar, Samantha, Sampson, Maureen McGuirk, Koopmans, Frank, Prjibelski, Andrey D., Mikheenko, Alla, Belchikov, Natan, Jarroux, Julien, Lucas, Anne Bergstrom, Palkovits, Miklós, Luo, Wenjie, Milner, Teresa A., Ndhlovu, Lishomwa C., Smit, August B., Trojanowski, John Q., Lee, Virginia M. Y., Fedrigo, Olivier, Sloan, Steven A., Tombácz, Dóra, Ross, M. Elizabeth, Jarvis, Erich, Boldogkői, Zsolt, Gan, Li, Tilgner, Hagen U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287170/
https://www.ncbi.nlm.nih.gov/pubmed/35256815
http://dx.doi.org/10.1038/s41587-022-01231-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287170/
https://www.ncbi.nlm.nih.gov/pubmed/35256815
http://dx.doi.org/10.1038/s41587-022-01231-3

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