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The disconnect2 mutation disrupts the tjp1b gene that is required for electrical synapse formation

To investigate electrical synapse formation in vivo we used forward genetics to disrupt genes affecting Mauthner cell electrical synapses in larval zebrafish. We identify the disconnect2 ( dis2 ) mutation for its failure to localize neural gap junction channels at electrical synapses. We mapped this...

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Detalles Bibliográficos
Autores principales: Michel, Jennifer Carlisle, Lasseigne, Abagael M., Marsh, Audrey J., Miller, Adam C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288654/
https://www.ncbi.nlm.nih.gov/pubmed/35855444
http://dx.doi.org/10.17912/micropub.biology.000593
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author Michel, Jennifer Carlisle
Lasseigne, Abagael M.
Marsh, Audrey J.
Miller, Adam C.
author_facet Michel, Jennifer Carlisle
Lasseigne, Abagael M.
Marsh, Audrey J.
Miller, Adam C.
author_sort Michel, Jennifer Carlisle
collection PubMed
description To investigate electrical synapse formation in vivo we used forward genetics to disrupt genes affecting Mauthner cell electrical synapses in larval zebrafish. We identify the disconnect2 ( dis2 ) mutation for its failure to localize neural gap junction channels at electrical synapses. We mapped this mutation to chromosome 25 and identified a splice-altering mutation in the tjp1b gene. We demonstrated that the dis2 mutation disrupts tjp1b function using complementation analysis with CRISPR generated mutants. We conclude that the dis2 mutation disrupts the tjp1b gene that is required for electrical synapse formation.
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spelling pubmed-92886542022-07-18 The disconnect2 mutation disrupts the tjp1b gene that is required for electrical synapse formation Michel, Jennifer Carlisle Lasseigne, Abagael M. Marsh, Audrey J. Miller, Adam C. MicroPubl Biol New Finding To investigate electrical synapse formation in vivo we used forward genetics to disrupt genes affecting Mauthner cell electrical synapses in larval zebrafish. We identify the disconnect2 ( dis2 ) mutation for its failure to localize neural gap junction channels at electrical synapses. We mapped this mutation to chromosome 25 and identified a splice-altering mutation in the tjp1b gene. We demonstrated that the dis2 mutation disrupts tjp1b function using complementation analysis with CRISPR generated mutants. We conclude that the dis2 mutation disrupts the tjp1b gene that is required for electrical synapse formation. Caltech Library 2022-07-03 /pmc/articles/PMC9288654/ /pubmed/35855444 http://dx.doi.org/10.17912/micropub.biology.000593 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Michel, Jennifer Carlisle
Lasseigne, Abagael M.
Marsh, Audrey J.
Miller, Adam C.
The disconnect2 mutation disrupts the tjp1b gene that is required for electrical synapse formation
title The disconnect2 mutation disrupts the tjp1b gene that is required for electrical synapse formation
title_full The disconnect2 mutation disrupts the tjp1b gene that is required for electrical synapse formation
title_fullStr The disconnect2 mutation disrupts the tjp1b gene that is required for electrical synapse formation
title_full_unstemmed The disconnect2 mutation disrupts the tjp1b gene that is required for electrical synapse formation
title_short The disconnect2 mutation disrupts the tjp1b gene that is required for electrical synapse formation
title_sort the disconnect2 mutation disrupts the tjp1b gene that is required for electrical synapse formation
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288654/
https://www.ncbi.nlm.nih.gov/pubmed/35855444
http://dx.doi.org/10.17912/micropub.biology.000593
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