Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area

Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD(260)). Here, we describe a method for direct dete...

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Detalles Bibliográficos
Autores principales: Chillar, Komal, Yin, Yipeng, Eriyagama, Adikari M. Dhananjani N., Fang, Shiyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288849/
https://www.ncbi.nlm.nih.gov/pubmed/35856072
http://dx.doi.org/10.7717/peerj-achem.20

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288849/
https://www.ncbi.nlm.nih.gov/pubmed/35856072
http://dx.doi.org/10.7717/peerj-achem.20

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