Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area

Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD(260)). Here, we describe a method for direct dete...

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Detalles Bibliográficos
Autores principales: Chillar, Komal, Yin, Yipeng, Eriyagama, Adikari M. Dhananjani N., Fang, Shiyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288849/
https://www.ncbi.nlm.nih.gov/pubmed/35856072
http://dx.doi.org/10.7717/peerj-achem.20
Descripción
Sumario:Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD(260)). Here, we describe a method for direct determination of OD(260) of ODNs using the area of the peaks in HPLC profiles. It is expected that the method will save significant time for researchers in the area of nucleic acid research, and minimize the loss of oligonucleotide samples.

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