Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area

Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD(260)). Here, we describe a method for direct dete...

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Autores principales: Chillar, Komal, Yin, Yipeng, Eriyagama, Adikari M. Dhananjani N., Fang, Shiyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288849/
https://www.ncbi.nlm.nih.gov/pubmed/35856072
http://dx.doi.org/10.7717/peerj-achem.20
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author Chillar, Komal
Yin, Yipeng
Eriyagama, Adikari M. Dhananjani N.
Fang, Shiyue
author_facet Chillar, Komal
Yin, Yipeng
Eriyagama, Adikari M. Dhananjani N.
Fang, Shiyue
author_sort Chillar, Komal
collection PubMed
description Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD(260)). Here, we describe a method for direct determination of OD(260) of ODNs using the area of the peaks in HPLC profiles. It is expected that the method will save significant time for researchers in the area of nucleic acid research, and minimize the loss of oligonucleotide samples.
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spelling pubmed-92888492022-07-17 Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area Chillar, Komal Yin, Yipeng Eriyagama, Adikari M. Dhananjani N. Fang, Shiyue PeerJ Anal Chem Article Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD(260)). Here, we describe a method for direct determination of OD(260) of ODNs using the area of the peaks in HPLC profiles. It is expected that the method will save significant time for researchers in the area of nucleic acid research, and minimize the loss of oligonucleotide samples. 2022 2022-07-07 /pmc/articles/PMC9288849/ /pubmed/35856072 http://dx.doi.org/10.7717/peerj-achem.20 Text en https://creativecommons.org/licenses/by/4.0/ Distributed under Creative Commons CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/)
spellingShingle Article
Chillar, Komal
Yin, Yipeng
Eriyagama, Adikari M. Dhananjani N.
Fang, Shiyue
Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area
title Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area
title_full Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area
title_fullStr Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area
title_full_unstemmed Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area
title_short Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area
title_sort determination of optical density (od) of oligodeoxynucleotide from hplc peak area
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288849/
https://www.ncbi.nlm.nih.gov/pubmed/35856072
http://dx.doi.org/10.7717/peerj-achem.20
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AT yinyipeng determinationofopticaldensityodofoligodeoxynucleotidefromhplcpeakarea
AT eriyagamaadikarimdhananjanin determinationofopticaldensityodofoligodeoxynucleotidefromhplcpeakarea
AT fangshiyue determinationofopticaldensityodofoligodeoxynucleotidefromhplcpeakarea

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