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Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area
Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD(260)). Here, we describe a method for direct dete...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288849/ https://www.ncbi.nlm.nih.gov/pubmed/35856072 http://dx.doi.org/10.7717/peerj-achem.20 |