Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex‐dependent manner

Monocytes play a critical role in inflammation and immune response, their activity being sex‐dependent. However, the basis of sex differences is not well understood. Therefore, we investigated the lipopolysaccharide (LPS) effects on tumor necrosis factor‐α (TNF‐α) release, autophagy, and chemotaxis...

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Autores principales: Campesi, Ilaria, Montella, Andrea, Franconi, Flavia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9292909/
https://www.ncbi.nlm.nih.gov/pubmed/34252202
http://dx.doi.org/10.1002/jcp.30503
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author Campesi, Ilaria
Montella, Andrea
Franconi, Flavia
author_facet Campesi, Ilaria
Montella, Andrea
Franconi, Flavia
author_sort Campesi, Ilaria
collection PubMed
description Monocytes play a critical role in inflammation and immune response, their activity being sex‐dependent. However, the basis of sex differences is not well understood. Therefore, we investigated the lipopolysaccharide (LPS) effects on tumor necrosis factor‐α (TNF‐α) release, autophagy, and chemotaxis in freshly isolated monocytes from healthy young men and women. In basal conditions, male and female monocytes had similar TNF‐α release, chemotaxis, and estrogen receptors (ER‐α) and ER‐β expression, while the LC3II/I ratio was significantly higher in males. LPS treatment induced qualitative and quantitative sex differences. It reduced autophagy and increased TNF‐α release only in male monocytes, while, chemotaxis was significantly influenced only in female cells. Moreover, it reduced the expression of ER‐α only in female cells, while ER‐β expression was reduced in both sexes, but more markedly in female cells. Finally, the interplay between LPS treatment and 17‐β‐estradiol (E(2)) was present only in female cells. Globally, these findings expand the concept that sex plays a role in regulating monocytes' functions, being sex differences cell‐ and parameter‐specific.
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spelling pubmed-92929092022-07-20 Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex‐dependent manner Campesi, Ilaria Montella, Andrea Franconi, Flavia J Cell Physiol Research Articles Monocytes play a critical role in inflammation and immune response, their activity being sex‐dependent. However, the basis of sex differences is not well understood. Therefore, we investigated the lipopolysaccharide (LPS) effects on tumor necrosis factor‐α (TNF‐α) release, autophagy, and chemotaxis in freshly isolated monocytes from healthy young men and women. In basal conditions, male and female monocytes had similar TNF‐α release, chemotaxis, and estrogen receptors (ER‐α) and ER‐β expression, while the LC3II/I ratio was significantly higher in males. LPS treatment induced qualitative and quantitative sex differences. It reduced autophagy and increased TNF‐α release only in male monocytes, while, chemotaxis was significantly influenced only in female cells. Moreover, it reduced the expression of ER‐α only in female cells, while ER‐β expression was reduced in both sexes, but more markedly in female cells. Finally, the interplay between LPS treatment and 17‐β‐estradiol (E(2)) was present only in female cells. Globally, these findings expand the concept that sex plays a role in regulating monocytes' functions, being sex differences cell‐ and parameter‐specific. John Wiley and Sons Inc. 2021-07-12 2022-01 /pmc/articles/PMC9292909/ /pubmed/34252202 http://dx.doi.org/10.1002/jcp.30503 Text en © 2021 The Authors. Journal of Cellular Physiology published by Wiley Periodicals LLC https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Campesi, Ilaria
Montella, Andrea
Franconi, Flavia
Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex‐dependent manner
title Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex‐dependent manner
title_full Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex‐dependent manner
title_fullStr Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex‐dependent manner
title_full_unstemmed Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex‐dependent manner
title_short Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex‐dependent manner
title_sort human monocytes respond to lipopolysaccharide (lps) stimulation in a sex‐dependent manner
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9292909/
https://www.ncbi.nlm.nih.gov/pubmed/34252202
http://dx.doi.org/10.1002/jcp.30503
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