Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex‐dependent manner
Monocytes play a critical role in inflammation and immune response, their activity being sex‐dependent. However, the basis of sex differences is not well understood. Therefore, we investigated the lipopolysaccharide (LPS) effects on tumor necrosis factor‐α (TNF‐α) release, autophagy, and chemotaxis...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9292909/ https://www.ncbi.nlm.nih.gov/pubmed/34252202 http://dx.doi.org/10.1002/jcp.30503 |
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author | Campesi, Ilaria Montella, Andrea Franconi, Flavia |
author_facet | Campesi, Ilaria Montella, Andrea Franconi, Flavia |
author_sort | Campesi, Ilaria |
collection | PubMed |
description | Monocytes play a critical role in inflammation and immune response, their activity being sex‐dependent. However, the basis of sex differences is not well understood. Therefore, we investigated the lipopolysaccharide (LPS) effects on tumor necrosis factor‐α (TNF‐α) release, autophagy, and chemotaxis in freshly isolated monocytes from healthy young men and women. In basal conditions, male and female monocytes had similar TNF‐α release, chemotaxis, and estrogen receptors (ER‐α) and ER‐β expression, while the LC3II/I ratio was significantly higher in males. LPS treatment induced qualitative and quantitative sex differences. It reduced autophagy and increased TNF‐α release only in male monocytes, while, chemotaxis was significantly influenced only in female cells. Moreover, it reduced the expression of ER‐α only in female cells, while ER‐β expression was reduced in both sexes, but more markedly in female cells. Finally, the interplay between LPS treatment and 17‐β‐estradiol (E(2)) was present only in female cells. Globally, these findings expand the concept that sex plays a role in regulating monocytes' functions, being sex differences cell‐ and parameter‐specific. |
format | Online Article Text |
id | pubmed-9292909 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92929092022-07-20 Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex‐dependent manner Campesi, Ilaria Montella, Andrea Franconi, Flavia J Cell Physiol Research Articles Monocytes play a critical role in inflammation and immune response, their activity being sex‐dependent. However, the basis of sex differences is not well understood. Therefore, we investigated the lipopolysaccharide (LPS) effects on tumor necrosis factor‐α (TNF‐α) release, autophagy, and chemotaxis in freshly isolated monocytes from healthy young men and women. In basal conditions, male and female monocytes had similar TNF‐α release, chemotaxis, and estrogen receptors (ER‐α) and ER‐β expression, while the LC3II/I ratio was significantly higher in males. LPS treatment induced qualitative and quantitative sex differences. It reduced autophagy and increased TNF‐α release only in male monocytes, while, chemotaxis was significantly influenced only in female cells. Moreover, it reduced the expression of ER‐α only in female cells, while ER‐β expression was reduced in both sexes, but more markedly in female cells. Finally, the interplay between LPS treatment and 17‐β‐estradiol (E(2)) was present only in female cells. Globally, these findings expand the concept that sex plays a role in regulating monocytes' functions, being sex differences cell‐ and parameter‐specific. John Wiley and Sons Inc. 2021-07-12 2022-01 /pmc/articles/PMC9292909/ /pubmed/34252202 http://dx.doi.org/10.1002/jcp.30503 Text en © 2021 The Authors. Journal of Cellular Physiology published by Wiley Periodicals LLC https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Campesi, Ilaria Montella, Andrea Franconi, Flavia Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex‐dependent manner |
title | Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex‐dependent manner |
title_full | Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex‐dependent manner |
title_fullStr | Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex‐dependent manner |
title_full_unstemmed | Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex‐dependent manner |
title_short | Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex‐dependent manner |
title_sort | human monocytes respond to lipopolysaccharide (lps) stimulation in a sex‐dependent manner |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9292909/ https://www.ncbi.nlm.nih.gov/pubmed/34252202 http://dx.doi.org/10.1002/jcp.30503 |
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