Cryo-EM structures reveal that RFC recognizes both the 3′- and 5′-DNA ends to load PCNA onto gaps for DNA repair

RFC uses ATP to assemble PCNA onto primed sites for replicative DNA polymerases δ and ε. The RFC pentamer forms a central chamber that binds 3′ ss/ds DNA junctions to load PCNA onto DNA during replication. We show here five structures that identify a second DNA binding site in RFC that binds a 5′ du...

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Detalles Bibliográficos
Autores principales: Zheng, Fengwei, Georgescu, Roxana, Yao, Nina Y, Li, Huilin, O'Donnell, Michael E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Biochemistry and Chemical Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293004/
https://www.ncbi.nlm.nih.gov/pubmed/35829698
http://dx.doi.org/10.7554/eLife.77469

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293004/
https://www.ncbi.nlm.nih.gov/pubmed/35829698
http://dx.doi.org/10.7554/eLife.77469

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