Optimized protocol for isolation of germ cells from mouse testis by centrifugal elutriation

Germline development is challenging to study due to the diversity of cell types in mammalian testis. Here, we present an optimized protocol, namely centrifugal elutriation, that allows the simultaneous isolation of mouse germ cells at different stages with high purity within ∼2 h. This approach expl...

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Detalles Bibliográficos
Autores principales: Cao, Yuzhu, Meng, Lan, Cheng, Yu, Azhar, Muhammad, Jiang, Xue, Xing, Xuemei, Bao, Jianqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293666/
https://www.ncbi.nlm.nih.gov/pubmed/35834386
http://dx.doi.org/10.1016/j.xpro.2022.101540

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293666/
https://www.ncbi.nlm.nih.gov/pubmed/35834386
http://dx.doi.org/10.1016/j.xpro.2022.101540

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