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Production of human A(2A)AR in lipid nanodiscs for (19)F-NMR and single-molecule fluorescence spectroscopy
We describe production of the human A(2A) adenosine receptor (A(2A)AR), a class A G protein-coupled receptor (GPCR) for (19)F-NMR and single-molecule fluorescence (SMF) spectroscopy. We explain in detail steps shared between the two sample preparation strategies, including expression and isolation o...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293669/ https://www.ncbi.nlm.nih.gov/pubmed/35839771 http://dx.doi.org/10.1016/j.xpro.2022.101535 |