Production of human A(2A)AR in lipid nanodiscs for (19)F-NMR and single-molecule fluorescence spectroscopy

We describe production of the human A(2A) adenosine receptor (A(2A)AR), a class A G protein-coupled receptor (GPCR) for (19)F-NMR and single-molecule fluorescence (SMF) spectroscopy. We explain in detail steps shared between the two sample preparation strategies, including expression and isolation o...

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Detalles Bibliográficos
Autores principales: Thakur, Naveen, Wei, Shushu, Ray, Arka Prabha, Lamichhane, Rajan, Eddy, Matthew T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293669/
https://www.ncbi.nlm.nih.gov/pubmed/35839771
http://dx.doi.org/10.1016/j.xpro.2022.101535

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