Using human iPSC-derived kidney organoids to decipher SARS-CoV-2 pathology on single cell level

We describe a protocol for single-cell RNA sequencing of SARS-CoV-2-infected human induced pluripotent stem cell (iPSC)-derived kidney organoids. After inoculation of kidney organoids with virus, we use mechanical and enzymatic disruption to obtain single cell suspensions. Next, we process the organ...

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Detalles Bibliográficos
Autores principales: Reimer, Katharina C., Jansen, Jitske, Overheul, Gijs J., Miesen, Pascal, van Rij, Ronald P., Triana, Sergio H., Smeets, Bart, Schneider, Rebekka K., Kramann, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293950/
https://www.ncbi.nlm.nih.gov/pubmed/35983169
http://dx.doi.org/10.1016/j.xpro.2022.101612

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293950/
https://www.ncbi.nlm.nih.gov/pubmed/35983169
http://dx.doi.org/10.1016/j.xpro.2022.101612

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