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Molecular tension microscopy of the LINC complex in live cells

We present a protocol to measure the effect of pharmacological treatments on the mechanical tension experienced by nesprins at the cytoplasmic surface of the nuclear envelope of mammalian cells in culture. We apply this protocol to MDCK epithelial cells exposed to the actin depolymerization agent cy...

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Detalles Bibliográficos
Autores principales: Sipieter, François, Laurent, Louis, Girard, Philippe P., Borghi, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294191/
https://www.ncbi.nlm.nih.gov/pubmed/35841591
http://dx.doi.org/10.1016/j.xpro.2022.101538