Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systems

Our recent development of the CRAGE (chassis-independent recombinase-assisted genome engineering) system enables single-step integration of large, complex DNA constructs directly into bacteria genomes across multiple phyla. This protocol describes the details of the experimental design and procedure...

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Detalles Bibliográficos
Autores principales: Zhao, Zhiying, Cheng, Jan-Fang, Yoshikuni, Yasuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294251/
https://www.ncbi.nlm.nih.gov/pubmed/35842866
http://dx.doi.org/10.1016/j.xpro.2022.101546

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294251/
https://www.ncbi.nlm.nih.gov/pubmed/35842866
http://dx.doi.org/10.1016/j.xpro.2022.101546

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