Cargando…
Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systems
Our recent development of the CRAGE (chassis-independent recombinase-assisted genome engineering) system enables single-step integration of large, complex DNA constructs directly into bacteria genomes across multiple phyla. This protocol describes the details of the experimental design and procedure...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294251/ https://www.ncbi.nlm.nih.gov/pubmed/35842866 http://dx.doi.org/10.1016/j.xpro.2022.101546 |