Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells

Male germ-cell development comprises primordial germ-cell (PGC) development, spermatogonium differentiation, and ensuing spermatogenesis. We present a step-by-step protocol for differentiation of mouse pluripotent stem cells (PSCs) into germline stem-cell-like cells (GSCLCs) via PGC-like cell and sp...

Descripción completa

Detalles Bibliográficos
Autores principales: Ishikura, Yukiko, Ohta, Hiroshi, Nagano, Masahiro, Saitou, Mitinori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294266/
https://www.ncbi.nlm.nih.gov/pubmed/35842863
http://dx.doi.org/10.1016/j.xpro.2022.101544

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294266/
https://www.ncbi.nlm.nih.gov/pubmed/35842863
http://dx.doi.org/10.1016/j.xpro.2022.101544

Ejemplares similares