Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells

Male germ-cell development comprises primordial germ-cell (PGC) development, spermatogonium differentiation, and ensuing spermatogenesis. We present a step-by-step protocol for differentiation of mouse pluripotent stem cells (PSCs) into germline stem-cell-like cells (GSCLCs) via PGC-like cell and sp...

Descripción completa

Detalles Bibliográficos
Autores principales: Ishikura, Yukiko, Ohta, Hiroshi, Nagano, Masahiro, Saitou, Mitinori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294266/
https://www.ncbi.nlm.nih.gov/pubmed/35842863
http://dx.doi.org/10.1016/j.xpro.2022.101544
_version_ 1784749812496728064
author Ishikura, Yukiko
Ohta, Hiroshi
Nagano, Masahiro
Saitou, Mitinori
author_facet Ishikura, Yukiko
Ohta, Hiroshi
Nagano, Masahiro
Saitou, Mitinori
author_sort Ishikura, Yukiko
collection PubMed
description Male germ-cell development comprises primordial germ-cell (PGC) development, spermatogonium differentiation, and ensuing spermatogenesis. We present a step-by-step protocol for differentiation of mouse pluripotent stem cells (PSCs) into germline stem-cell-like cells (GSCLCs) via PGC-like cell and spermatogonium-like cell intermediates. The differentiation protocol has higher fidelity than our previous protocol. Upon transplantation into testes in vivo or culture for testis transplants, GSCLCs robustly contribute to spermatogenesis, providing a paradigm for PSC-based reconstitution of mammalian male germ-cell development. For complete details on the use and execution of this protocol, please refer to Ishikura et al. (2021).
format Online
Article
Text
id pubmed-9294266
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-92942662022-07-20 Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells Ishikura, Yukiko Ohta, Hiroshi Nagano, Masahiro Saitou, Mitinori STAR Protoc Protocol Male germ-cell development comprises primordial germ-cell (PGC) development, spermatogonium differentiation, and ensuing spermatogenesis. We present a step-by-step protocol for differentiation of mouse pluripotent stem cells (PSCs) into germline stem-cell-like cells (GSCLCs) via PGC-like cell and spermatogonium-like cell intermediates. The differentiation protocol has higher fidelity than our previous protocol. Upon transplantation into testes in vivo or culture for testis transplants, GSCLCs robustly contribute to spermatogenesis, providing a paradigm for PSC-based reconstitution of mammalian male germ-cell development. For complete details on the use and execution of this protocol, please refer to Ishikura et al. (2021). Elsevier 2022-07-16 /pmc/articles/PMC9294266/ /pubmed/35842863 http://dx.doi.org/10.1016/j.xpro.2022.101544 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ishikura, Yukiko
Ohta, Hiroshi
Nagano, Masahiro
Saitou, Mitinori
Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells
title Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells
title_full Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells
title_fullStr Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells
title_full_unstemmed Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells
title_short Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells
title_sort optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294266/
https://www.ncbi.nlm.nih.gov/pubmed/35842863
http://dx.doi.org/10.1016/j.xpro.2022.101544
work_keys_str_mv AT ishikurayukiko optimizedprotocoltoderivegermlinestemcelllikecellsfrommousepluripotentstemcells
AT ohtahiroshi optimizedprotocoltoderivegermlinestemcelllikecellsfrommousepluripotentstemcells
AT naganomasahiro optimizedprotocoltoderivegermlinestemcelllikecellsfrommousepluripotentstemcells
AT saitoumitinori optimizedprotocoltoderivegermlinestemcelllikecellsfrommousepluripotentstemcells

Ejemplares similares