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Protocol to assess cell-intrinsic regulatory mechanisms using an ex vivo murine T cell polarization and co-culture system
This protocol describes an ex vivo cell culture system for simultaneously generating a mixture of CD4(+) T helper lineages, including T helper 17 (Th17), RORγt(+) Treg, and conventional Treg (cTreg), in proportions representative of those found in mucosal tissues in vivo. When combined with a co-cul...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294555/ https://www.ncbi.nlm.nih.gov/pubmed/35839772 http://dx.doi.org/10.1016/j.xpro.2022.101543 |