Insight into the broadened substrate scope of nitrile hydratase by static and dynamic structure analysis

The narrow substrate scope limits the wide industrial application of enzymes. Here, we successfully broadened the substrate scope of a nitrile hydratase (NHase) through mutation of two tunnel entrance residues based on rational tunnel calculation. Two variants, with increased specific activity, espe...

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Detalles Bibliográficos
Autores principales: Ma, Dong, Cheng, Zhongyi, Peplowski, Lukasz, Han, Laichuang, Xia, Yuanyuan, Hou, Xiaodong, Guo, Junling, Yin, Dejing, Rao, Yijian, Zhou, Zhemin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297474/
https://www.ncbi.nlm.nih.gov/pubmed/35919716
http://dx.doi.org/10.1039/d2sc02319a

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297474/
https://www.ncbi.nlm.nih.gov/pubmed/35919716
http://dx.doi.org/10.1039/d2sc02319a

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