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qRAT: an R-based stand-alone application for relative expression analysis of RT-qPCR data

BACKGROUND: Reverse transcription quantitative real-time PCR (RT-qPCR) is a well-established method for analysing gene expression. Most RT-qPCR experiments in the field of microbiology aim for the detection of transcriptional changes by relative quantification, which means the comparison of the expr...

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Detalles Bibliográficos
Autores principales: Flatschacher, Daniel, Speckbacher, Verena, Zeilinger, Susanne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297597/
https://www.ncbi.nlm.nih.gov/pubmed/35854213
http://dx.doi.org/10.1186/s12859-022-04823-7