HaloTag Engineering for Enhanced Fluorogenicity and Kinetics with a Styrylpyridium Dye

HaloTag is a small self‐labeling protein that is frequently used for creating fluorescent reporters in living cells. The small‐molecule dyes used with HaloTag are almost exclusively based on rhodamine scaffolds, which are often expensive and challenging to synthesize. Herein, we report the engineeri...

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Detalles Bibliográficos
Autores principales: Miró‐Vinyals, Carla, Stein, Alina, Fischer, Sandro, Ward, Thomas R., Deliz Liang, Alexandria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298263/
https://www.ncbi.nlm.nih.gov/pubmed/34609782
http://dx.doi.org/10.1002/cbic.202100424

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298263/
https://www.ncbi.nlm.nih.gov/pubmed/34609782
http://dx.doi.org/10.1002/cbic.202100424

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