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HaloTag Engineering for Enhanced Fluorogenicity and Kinetics with a Styrylpyridium Dye
HaloTag is a small self‐labeling protein that is frequently used for creating fluorescent reporters in living cells. The small‐molecule dyes used with HaloTag are almost exclusively based on rhodamine scaffolds, which are often expensive and challenging to synthesize. Herein, we report the engineeri...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298263/ https://www.ncbi.nlm.nih.gov/pubmed/34609782 http://dx.doi.org/10.1002/cbic.202100424 |