Strong and Specific Recognition of CAG/CTG Repeat DNA (5’‐dWGCWGCW‐3’) by a Cyclic Pyrrole‐Imidazole Polyamide

Abnormally expanded CAG/CTG repeat DNA sequences lead to a variety of neurological diseases, such as Huntington's disease. Here, we synthesized a cyclic pyrrole‐imidazole polyamide (cPIP), which can bind to the minor groove of the CAG/CTG DNA sequence. The double‐stranded DNA melting temperatur...

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Autores principales: Hirose, Yuki, Ohno, Tomo, Asamitsu, Sefan, Hashiya, Kaori, Bando, Toshikazu, Sugiyama, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298716/
https://www.ncbi.nlm.nih.gov/pubmed/34796607
http://dx.doi.org/10.1002/cbic.202100533
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author Hirose, Yuki
Ohno, Tomo
Asamitsu, Sefan
Hashiya, Kaori
Bando, Toshikazu
Sugiyama, Hiroshi
author_facet Hirose, Yuki
Ohno, Tomo
Asamitsu, Sefan
Hashiya, Kaori
Bando, Toshikazu
Sugiyama, Hiroshi
author_sort Hirose, Yuki
collection PubMed
description Abnormally expanded CAG/CTG repeat DNA sequences lead to a variety of neurological diseases, such as Huntington's disease. Here, we synthesized a cyclic pyrrole‐imidazole polyamide (cPIP), which can bind to the minor groove of the CAG/CTG DNA sequence. The double‐stranded DNA melting temperature (T (m)) and surface plasmon resonance assays revealed the high binding affinity of the cPIP. In addition, next‐generation sequencing showed that the cPIP had high specificity for its target DNA sequence.
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spelling pubmed-92987162022-07-21 Strong and Specific Recognition of CAG/CTG Repeat DNA (5’‐dWGCWGCW‐3’) by a Cyclic Pyrrole‐Imidazole Polyamide Hirose, Yuki Ohno, Tomo Asamitsu, Sefan Hashiya, Kaori Bando, Toshikazu Sugiyama, Hiroshi Chembiochem Research Articles Abnormally expanded CAG/CTG repeat DNA sequences lead to a variety of neurological diseases, such as Huntington's disease. Here, we synthesized a cyclic pyrrole‐imidazole polyamide (cPIP), which can bind to the minor groove of the CAG/CTG DNA sequence. The double‐stranded DNA melting temperature (T (m)) and surface plasmon resonance assays revealed the high binding affinity of the cPIP. In addition, next‐generation sequencing showed that the cPIP had high specificity for its target DNA sequence. John Wiley and Sons Inc. 2021-11-18 2022-01-19 /pmc/articles/PMC9298716/ /pubmed/34796607 http://dx.doi.org/10.1002/cbic.202100533 Text en © 2021 The Authors. ChemBioChem published by Wiley-VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Hirose, Yuki
Ohno, Tomo
Asamitsu, Sefan
Hashiya, Kaori
Bando, Toshikazu
Sugiyama, Hiroshi
Strong and Specific Recognition of CAG/CTG Repeat DNA (5’‐dWGCWGCW‐3’) by a Cyclic Pyrrole‐Imidazole Polyamide
title Strong and Specific Recognition of CAG/CTG Repeat DNA (5’‐dWGCWGCW‐3’) by a Cyclic Pyrrole‐Imidazole Polyamide
title_full Strong and Specific Recognition of CAG/CTG Repeat DNA (5’‐dWGCWGCW‐3’) by a Cyclic Pyrrole‐Imidazole Polyamide
title_fullStr Strong and Specific Recognition of CAG/CTG Repeat DNA (5’‐dWGCWGCW‐3’) by a Cyclic Pyrrole‐Imidazole Polyamide
title_full_unstemmed Strong and Specific Recognition of CAG/CTG Repeat DNA (5’‐dWGCWGCW‐3’) by a Cyclic Pyrrole‐Imidazole Polyamide
title_short Strong and Specific Recognition of CAG/CTG Repeat DNA (5’‐dWGCWGCW‐3’) by a Cyclic Pyrrole‐Imidazole Polyamide
title_sort strong and specific recognition of cag/ctg repeat dna (5’‐dwgcwgcw‐3’) by a cyclic pyrrole‐imidazole polyamide
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298716/
https://www.ncbi.nlm.nih.gov/pubmed/34796607
http://dx.doi.org/10.1002/cbic.202100533
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