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Digital holography-based 3D particle localization for single-molecule tweezer techniques

We present a three-dimensional (3D) imaging technique for the fast tracking of microscopic objects in a fluid environment. Our technique couples digital holographic microscopy with three-dimensional localization via parabolic masking. Compared with existing approaches, our method reconstructs 3D vol...

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Detalles Bibliográficos
Autores principales: Flewellen, James L., Minoughan, Sophie, Garcia, Isabel Llorente, Tolar, Pavel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300656/
https://www.ncbi.nlm.nih.gov/pubmed/35659644
http://dx.doi.org/10.1016/j.bpj.2022.06.001