Direct adenylation from 5′-OH-terminated oligonucleotides by a fusion enzyme containing Pfu RNA ligase and T4 polynucleotide kinase

5′-Adenylated oligonucleotides (AppOligos) are widely used for single-stranded DNA/RNA ligation in next-generation sequencing (NGS) applications such as microRNA (miRNA) profiling. The ligation between an AppOligo adapter and target molecules (such as miRNA) no longer requires ATP, thereby minimizin...

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Detalles Bibliográficos
Autores principales: Yang, Zhengquan, Zhang, Chengliang, Lian, Guojun, Dong, Shijie, Song, Menghui, Shao, Hengrong, Wang, Jingmei, Zhong, Tao, Luo, Zhenni, Jin, Shengnan, Ding, Chunming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303275/
https://www.ncbi.nlm.nih.gov/pubmed/35819229
http://dx.doi.org/10.1093/nar/gkac604

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303275/
https://www.ncbi.nlm.nih.gov/pubmed/35819229
http://dx.doi.org/10.1093/nar/gkac604

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