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Direct adenylation from 5′-OH-terminated oligonucleotides by a fusion enzyme containing Pfu RNA ligase and T4 polynucleotide kinase
5′-Adenylated oligonucleotides (AppOligos) are widely used for single-stranded DNA/RNA ligation in next-generation sequencing (NGS) applications such as microRNA (miRNA) profiling. The ligation between an AppOligo adapter and target molecules (such as miRNA) no longer requires ATP, thereby minimizin...
Autores principales: | Yang, Zhengquan, Zhang, Chengliang, Lian, Guojun, Dong, Shijie, Song, Menghui, Shao, Hengrong, Wang, Jingmei, Zhong, Tao, Luo, Zhenni, Jin, Shengnan, Ding, Chunming |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303275/ https://www.ncbi.nlm.nih.gov/pubmed/35819229 http://dx.doi.org/10.1093/nar/gkac604 |
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