Repurposing CRISPR RNA-guided integrases system for one-step, efficient genomic integration of ultra-long DNA sequences

Genomic integration techniques offer opportunities for generation of engineered microorganisms with improved or even entirely new functions but are currently limited by inability for efficient insertion of long genetic payloads due to multiplexing. Herein, using Shewanella oneidensis MR-1 as a model...

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Detalles Bibliográficos
Autores principales: Cheng, Zhou-Hua, Wu, Jie, Liu, Jia-Qi, Min, Di, Liu, Dong-Feng, Li, Wen-Wei, Yu, Han-Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303307/
https://www.ncbi.nlm.nih.gov/pubmed/35776123
http://dx.doi.org/10.1093/nar/gkac554

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303307/
https://www.ncbi.nlm.nih.gov/pubmed/35776123
http://dx.doi.org/10.1093/nar/gkac554

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