Cargando…

Circ_0018168 inhibits the proliferation and osteogenic differentiation of fibroblasts in ankylosing spondylitis via regulating miR-330-3p/DKK1 axis

BACKGROUND: Circular RNAs (circRNAs) play a crucial regulatory role in human diseases. However, the roles of circRNAs in ankylosing spondylitis (AS) are barely known. In this study, the functions of circ_0018168 in AS were investigated. METHODS: Quantitative real-time polymerase chain reaction (qRT-...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Lei, Jiao, Jiaxun, Yan, Guanghui, Wei, Wei, Fang, Genqiang, Yu, Tiemiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society for Regenerative Medicine 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304432/
https://www.ncbi.nlm.nih.gov/pubmed/35891711
http://dx.doi.org/10.1016/j.reth.2022.06.005
_version_ 1784752102755532800
author Zhao, Lei
Jiao, Jiaxun
Yan, Guanghui
Wei, Wei
Fang, Genqiang
Yu, Tiemiao
author_facet Zhao, Lei
Jiao, Jiaxun
Yan, Guanghui
Wei, Wei
Fang, Genqiang
Yu, Tiemiao
author_sort Zhao, Lei
collection PubMed
description BACKGROUND: Circular RNAs (circRNAs) play a crucial regulatory role in human diseases. However, the roles of circRNAs in ankylosing spondylitis (AS) are barely known. In this study, the functions of circ_0018168 in AS were investigated. METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) and western blot assay were used for circ_0018168, microRNA-330-3p (miR-330-3p), dickkopf-1 (DKK1), alkaline phosphatase (ALP), osteocalcin (OCN), Runt-related transcription factor 2 (Runx2) levels. Cell Counting Kit-8 (CCK-8) assay and 5′-ethynyl-2′-deoxyuridine (EdU) assay were conducted to analyze cell proliferation ability. Flow cytometry analysis was manipulated for cell cycle process. ALP activity was examined with a commercial kit. RNA immunoprecipitation (RIP) assay, RNA pull-down assay and dual-luciferase reporter assay were used to analyze the relationships of circ_0018168, miR-330-3p and DKK1. RESULTS: Circ_0018168 and DKK1 levels were lowly expressed in AS hip capsule specimens. Circ_0018168 overexpression repressed cell proliferation, cell cycle process as well as reduced ALP activity and ALP, OCN and Runx2 protein levels in AS fibroblasts. DKK1 silencing ameliorated the impact of circ_0018168 on AS progression. In addition, circ_0018168 served as the sponge for miR-330-3p, which could target DKK1. MiR-330-3p inhibition suppressed the proliferation, cell cycle and osteogenic differentiation in AS fibroblasts, but DKK1 silencing reversed the impacts. Besides, the effect of circ_0018168 on AS development was abolished by miR-330-3p upregulation. CONCLUSION: Circ_0018168 overexpression restrained fibroblast proliferation and osteogenic differentiation in AS by elevating DKK1 through adsorbing miR-330-3p.
format Online
Article
Text
id pubmed-9304432
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Japanese Society for Regenerative Medicine
record_format MEDLINE/PubMed
spelling pubmed-93044322022-07-25 Circ_0018168 inhibits the proliferation and osteogenic differentiation of fibroblasts in ankylosing spondylitis via regulating miR-330-3p/DKK1 axis Zhao, Lei Jiao, Jiaxun Yan, Guanghui Wei, Wei Fang, Genqiang Yu, Tiemiao Regen Ther Original Article BACKGROUND: Circular RNAs (circRNAs) play a crucial regulatory role in human diseases. However, the roles of circRNAs in ankylosing spondylitis (AS) are barely known. In this study, the functions of circ_0018168 in AS were investigated. METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) and western blot assay were used for circ_0018168, microRNA-330-3p (miR-330-3p), dickkopf-1 (DKK1), alkaline phosphatase (ALP), osteocalcin (OCN), Runt-related transcription factor 2 (Runx2) levels. Cell Counting Kit-8 (CCK-8) assay and 5′-ethynyl-2′-deoxyuridine (EdU) assay were conducted to analyze cell proliferation ability. Flow cytometry analysis was manipulated for cell cycle process. ALP activity was examined with a commercial kit. RNA immunoprecipitation (RIP) assay, RNA pull-down assay and dual-luciferase reporter assay were used to analyze the relationships of circ_0018168, miR-330-3p and DKK1. RESULTS: Circ_0018168 and DKK1 levels were lowly expressed in AS hip capsule specimens. Circ_0018168 overexpression repressed cell proliferation, cell cycle process as well as reduced ALP activity and ALP, OCN and Runx2 protein levels in AS fibroblasts. DKK1 silencing ameliorated the impact of circ_0018168 on AS progression. In addition, circ_0018168 served as the sponge for miR-330-3p, which could target DKK1. MiR-330-3p inhibition suppressed the proliferation, cell cycle and osteogenic differentiation in AS fibroblasts, but DKK1 silencing reversed the impacts. Besides, the effect of circ_0018168 on AS development was abolished by miR-330-3p upregulation. CONCLUSION: Circ_0018168 overexpression restrained fibroblast proliferation and osteogenic differentiation in AS by elevating DKK1 through adsorbing miR-330-3p. Japanese Society for Regenerative Medicine 2022-07-18 /pmc/articles/PMC9304432/ /pubmed/35891711 http://dx.doi.org/10.1016/j.reth.2022.06.005 Text en © 2022 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Zhao, Lei
Jiao, Jiaxun
Yan, Guanghui
Wei, Wei
Fang, Genqiang
Yu, Tiemiao
Circ_0018168 inhibits the proliferation and osteogenic differentiation of fibroblasts in ankylosing spondylitis via regulating miR-330-3p/DKK1 axis
title Circ_0018168 inhibits the proliferation and osteogenic differentiation of fibroblasts in ankylosing spondylitis via regulating miR-330-3p/DKK1 axis
title_full Circ_0018168 inhibits the proliferation and osteogenic differentiation of fibroblasts in ankylosing spondylitis via regulating miR-330-3p/DKK1 axis
title_fullStr Circ_0018168 inhibits the proliferation and osteogenic differentiation of fibroblasts in ankylosing spondylitis via regulating miR-330-3p/DKK1 axis
title_full_unstemmed Circ_0018168 inhibits the proliferation and osteogenic differentiation of fibroblasts in ankylosing spondylitis via regulating miR-330-3p/DKK1 axis
title_short Circ_0018168 inhibits the proliferation and osteogenic differentiation of fibroblasts in ankylosing spondylitis via regulating miR-330-3p/DKK1 axis
title_sort circ_0018168 inhibits the proliferation and osteogenic differentiation of fibroblasts in ankylosing spondylitis via regulating mir-330-3p/dkk1 axis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304432/
https://www.ncbi.nlm.nih.gov/pubmed/35891711
http://dx.doi.org/10.1016/j.reth.2022.06.005
work_keys_str_mv AT zhaolei circ0018168inhibitstheproliferationandosteogenicdifferentiationoffibroblastsinankylosingspondylitisviaregulatingmir3303pdkk1axis
AT jiaojiaxun circ0018168inhibitstheproliferationandosteogenicdifferentiationoffibroblastsinankylosingspondylitisviaregulatingmir3303pdkk1axis
AT yanguanghui circ0018168inhibitstheproliferationandosteogenicdifferentiationoffibroblastsinankylosingspondylitisviaregulatingmir3303pdkk1axis
AT weiwei circ0018168inhibitstheproliferationandosteogenicdifferentiationoffibroblastsinankylosingspondylitisviaregulatingmir3303pdkk1axis
AT fanggenqiang circ0018168inhibitstheproliferationandosteogenicdifferentiationoffibroblastsinankylosingspondylitisviaregulatingmir3303pdkk1axis
AT yutiemiao circ0018168inhibitstheproliferationandosteogenicdifferentiationoffibroblastsinankylosingspondylitisviaregulatingmir3303pdkk1axis