A protocol to measure slow protein dynamics of the cholera toxin B pentamers using broadband dielectric spectroscopy

The present protocol describes how to measure experimentally the slow protein dynamics that take place upon the thermal unfolding of the B subunit cholera toxin pentamers using broadband dielectric spectroscopy (BDS) in weakly hydrated and nanoconfined conditions. Transient unfolding intermediates,...

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Detalles Bibliográficos
Autores principales: Bourgeat, Laetitia, Pacini, Lorenza, Serghei, Anatoli, Lesieur, Claire
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304676/
https://www.ncbi.nlm.nih.gov/pubmed/35874473
http://dx.doi.org/10.1016/j.xpro.2022.101561

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304676/
https://www.ncbi.nlm.nih.gov/pubmed/35874473
http://dx.doi.org/10.1016/j.xpro.2022.101561

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